The study investigated the herbicide 2-4-D-amine effects on Barbus luteus Hamri fish. The carp were divided into two age categories and exposed to different concentrations of the pesticide. The study also measured water properties like water temperature, pH, EC, D.O., and hardness when the fish were exposed to the pesticide.

The study reveals that fish exposed to pesticides show surface symptoms like skin and gill bleeding, neck warping, and scale loss. Compared to a control sample, these fish show irritation or confusion. The acute toxicity (LC50) of the 2-4 D amine was determined for a period of 96 hours after the food was cut off (24 hours) prior to the experiment. The first category has a greater impact on fish than the second, as demonstrated by the statistical analysis results at the probability level (P<0.05), which indicated an inverse link between the pesticide toxicity and the fish age at usage.