This study was conducted to determine the impact of seasonal variations on the physicochemical and bacteriological parameters of spring water samples in Oji River Local Government Area of Enugu State, Nigeria. Water samples were collected in sterile plastic water containers at the point of discharge during the dry and wet seasons and analyzed using the standard methods for the examination of water and wastewater for physicochemical parameters. Bacteriological analyses were carried out using most probable number (MPN) method by multiple tube fermentation technique. The results showed that the colour (7-14.2 TCU); pH (7.18-8.4); temperature (24.6-28.1 oC); electrical conductivity in wet season (128.5-285.6 µs/cm); total dissolved solids (114.7-401 mg/L); total suspended solids (101.8-423.6 mg/L); total hardness (58-255 mg/L); total acidity (3.55-19.5 mg/L); total alkalinity (9.2-47 mg/L); biological oxygen demand (1.70-3.28 mg/L); nitrate (0.73-2.23 mg/L); ammonium in wet season (0.18-0.34 mg/L); zinc (0.011-0.085 mg/L); mercury (0.001-0.005 mg/L); copper (0.002-1.008 mg/L) and iron (0.031-0.318 mg/L) were within the WHO standard for drinking water quality while the phosphate (0.14-1.08 mg/L), lead (0.013-0.098 mg/L) and ammonium in dry season (0.48-0.75 mg/L) levels were not. The total viable counts exceeded the 1.0 x 102 cfu/mL WHO standard for drinking water and the MPN counts for total and faecal coliforms of (8-13 MPN/100 mL) and (0-2 MPN/100 mL) respectively also exceeded the WHO standard slightly. The isolated organisms were identified to be Escherichia coli, Salmonella spp., Shigella spp., Staphylococcus aureus and Vibrio cholerae. The presence of these pathogenic, indicator organisms in the water samples, as well as their physicochemical implications, render them unfit for drinking without adequate treatment. Liming and boiling treatment techniques are recommended.