The great threat to the purity of ground water is due to anthropogenic activities: industrial effluents, wastes from fertilizer garbage, chemical dumpsite, poultries and animal husbandries disinfectant, feed formulation, drugs disposed on land surface. These anthropogenic activities through chemical processes affect the unique nature of groundwater. The study therefore analyzed the chemical characterization of groundwater in selected areas of Port Harcourt Metropolis. The study area comprises 14 zones that make up Port Harcourt Metropolis. Two sampling points were purposively chosen in each of the zones, totaling 28 different boreholes sank from different parts of Port Harcourt metropolis. Analysis of pH, hardness, and chemical characteristics of groundwater were done using appropriate measures and standard procedures. The finding indicates that pH values of groundwater obtained in the study area is 0.005 which is less than 0.05 level of significance. This revealed that the groundwater in the study area is slightly acidic as all the water samples had a pH values that are less than 7 on the PH scale. Variation between the chemical properties indicated P-value of 0.000 which is less than 0.05 alpha level. The obtained result shows that there is no significant variation between the chemical properties of groundwater in the study area. The result further reveals that groundwater in the study area contain chemical properties of reasonable quantity. The study concludes that there should be regular monitoring on land to avert the discharge of harmful substances in the environment which might find its way to the groundwater and contaminate it.