Before going to large scale plantation of native tree species, optimal fertilization is required for production of quality tree seedlings in the nursery. This study explores the initial growth performance with different levels of inorganic fertilizers (Urea) applied on 9 months seedlings of Suregada multiflora and Firmiana colorata in the nursery of institute of Forestry and Environmental Sciences, University of Chittagong, Bangladesh during May 2017 to April 2018. Four treatments were applied for S. multiflora and five treatments for F. colorata in the nursery. The study showed that seedlings applied with urea (N) varied significantly (P<0.05) in comparison to control (i.e. unfertilized soil). Total length, number of leafs, sturdiness of S. multiflora was highest for 100 kg N ha^-1 application whereas the highest values for F. colorata were found when the seedlings treated with 300 kg N ha^-1. Similar trends of result found in shoot, root, fresh and dry biomass production for S. multiflora but for F. colorata, the values found highest in case of 400 kg N ha^-1 application. Maximum volume index and height increment of S. multiflora were recorded when the seedlings treated with 100 kg N ha^-1 but for F. colorata, the values found highest while 400 kg N ha^-1 applied in the seedlings. Therefore, the application of N at the rate of 400 kg ha^-1 for F. colorata (Udal) and 100 kg ha^-1 for S. multiflora (ban-naringa) may be recommended to boost up the height, diameter and seedling dry matter production in the nursery.